ECF59
ECF proteins

General description: ECF59 has homology to original ECF59 (100%) and is present in Planctomycetes (100%) order Planctomycetales. Proteins from this group have a linker ~10 amino acids longer.

Genomic context conservation: Original ECF59 is associated with protein kinases encoded in position +1 (Jogler et al., 2012). Indeed, we found an average of 0.94 protein kinase domains per ECF in ECF59. These kinases contain six transmembrane helixes in ECF59s1 (ECF59s1: 77.78%) and are fused to a phosphotransferase domain in 33.33% and an ABC-2 family transporter protein in 22.22%. Instead, the protein kinases of ECF59s2 usually contain one transmembrane helix (ECF59s2: 60%) and are fused to tetratricopeptide repeats in 20% of the cases. Interestingly, 40% of the protein encoded in +1 of ECF59s2 are tyrosine kinases, leaving opened if the rest of the protein kinases of ECF59 are also tyrosine kinases.

Promoter motif conservation: Predicted target promoter motifs include a conserved TC in -35.

Summary: In conclusion, ECF59 might be regulated by protein kinases, encoded in +1 in most of the cases, as in the case of original ECF59 (Jogler et al., 2012).


 


Basic information

Number of representative ECFs: 37

Number of non-redundant ECFs: 41

Sequences with C-terminal extension: 0.00%

Sequences with N-terminal extension: 2.44%

Overrepresented class: Planctomycetia [97.30%]



Sample Neighborhood

Protein SFJ61833.1 of Assembly GCA_900113665.1 (Planctomicrobium piriforme)


Promoter Motif



Figures

Protein sequence length distribution

Gene neighbourhood conservation analysis


Overall Pfam domain distribution: Cumulative frequency of Pfam domains across the genetic neighborhoods. Frequency is expressed as number of Pfam domains per ECF sigma factor. Only domains present in more than 75% of the neighborhoods are shown. Genetic neighborhoods contain the proteins encoded in ±10 from the ECF coding sequence. Only the non-overlapping, highest scoring domains are considered positive. If a protein contains several copies of a domain, only one instance is further considered. In order to avoid sequence bias, only proteins from assemblies defined as "representative" or "reference" by NCBI are included (see https://www.ncbi.nlm.nih.gov/assembly/help/).
Pfam domain distribution per position: Frequency of Pfam domain architectures in the proteins encoded in ±10 (x-axis) from the ECF coding sequences. Frequency is expressed as number of times a certain domain architecture appears per ECF sigma factor. Only the highest scoring domains with no position overlap are considered in the domain architectures. Note that the order of the Pfam domains in domain architectures may differ from their name. When a protein contains several copies of a domain, only one instance is further considered. Only domain architectures present in more than 20% of the proteins encoded in any position are shown. In order to avoid sequence bias, only proteins from assemblies defined as "representative" or "reference" by NCBI are included (see https://www.ncbi.nlm.nih.gov/assembly/help/).

Related publications

Title Journal Year Authors PubMed ECF groups
Identification of proteins likely to be involved in morphogenesis, cell division, and signal transduction in Planctomycetes by comparative genomics. Journal of bacteriology 2012 C. Jogler, J. Waldmann, X. Huang, M. Jogler, F. Glöckner, T. Mascher, R. Kolter PubMed: 23002222 ECF59, ECF46, ECF57, ECF62, ECF61, ECF217, ECF58
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