ECF54

ECF subgroups

ECF54s1 33 ECF54s2 31 ECF54s3 24 ECF54s4 16 ECF54s5 12 ECF54s6 9 ECF54s7 12 ECF54s8 11 ECF54s9 12 ECF54s10 10

ECF proteins

General description: Members of ECF54 are homologous to members of the original group ECF54 (100%) and are present in Actinobacteria (100%).

Genomic context conservation: Members of original ECF54 are encoded near carboxypeptidase-regulatory-like domains, subtilases and a CHAT domain protein with tetratricopeptide repeats (Huang et al., 2015). Indeed, a carboxypeptidase regulatory-like domain is present in an average of 0.61 copies per ECF always in position +1, a subtilase is found in an average of 0.68 copies per ECF in positions +2 or -1, CHAT domains are found in an average of 0.71 copies per ECF usually in positions +3 or-2, and tetratricopeptide repeats are found in an average of 1.98 per ECF usually in position +2 or -2. Given the conservation of these proteins and the lack of putative AS factor, they could take part in the regulation or response of members of ECF54, as suggested for original ECF54 (Huang et al., 2015).

Promoter motif conservation: Predicted target promoter motifs are only conserved in the largest subgroups (ECF54s2, ECF54s1, ECF54s3), with GTATCAG in -35 and CTCC in -10, in agreement with original ECF54 (Huang et al., 2015).

Summary: ECF54 preserves the characteristics of the original group ECF54 (Huang et al., 2015). Members of this group are usually encoded near subtilases, carboxypeptidase regulatory-like domains and CHAT domains, which are fused to tetratricopeptide repeats in some cases.