Anti-σ factor: Members of ECF33 might be regulated by putative AS factors encoded in +1 with one transmembrane helix (73.64%) and a zinc finger.
Genomic context conservation: Other than this protein, conserved domains in the genetic context of members of ECF33 include a subtilase (+2 of ECF33s3 and ECF33s2) and a binding-protein-dependent transport system inner membrane component (ECF33s3), which is usually present in the periplasmic transporters of ABC transport systems.
Promoter motif conservation: The target -35 element is extensively conserved (TGAACCTTT), and the -10 element varies amongst subgroups, although an AC is usually conserved. This promoter matches previous work on original ECF33 (Rhodius et al., 2013) and agrees with experimentally described target promoter of EcfQ (Masloboeva et al., 2012).
Summary: ECF33 might be regulated by zinc-finger AS factors located in position +1 with one TM helix (73.64%). Members of this group could be responsible for singlet oxygen resistance, as described for EcfQ of B. japonicum (Masloboeva et al., 2012). The conservation of the target promoter elements indicates that members of ECF33 usually are self-regulated.
Number of representative ECFs: 512
Number of non-redundant ECFs: 492
Sequences with C-terminal extension: 0.00%
Sequences with N-terminal extension: 1.63%
Overrepresented phylum: Proteobacteria [99.61%]
|The third pillar of bacterial signal transduction: classification of the extracytoplasmic function (ECF) sigma factor protein family.||Molecular microbiology||2009||A. Staroń, H. Sofia, S. Dietrich, L. Ulrich, H. Liesegang, T. Mascher||PubMed: 19737356||ECF103, ECF21, ECF123, ECF51, ECF39, ECF281, ECF102, ECF130, ECF122, ECF291, ECF15, ECF242, ECF22, ECF285, ECF106, ECF27, ECF31, ECF240, ECF114, ECF16, ECF38, ECF41, ECF105, ECF116, ECF111, ECF03, ECF239, ECF42, ECF294, ECF17, ECF11, ECF29, ECF235, ECF293, ECF118, ECF265, ECF30, ECF23, ECF14, ECF249, ECF18, ECF115, ECF290, ECF25, ECF121, ECF02, ECF120, ECF289, ECF28, ECF243, ECF19, ECF43, ECF107, ECF12, ECF32, ECF36, ECF292, ECF286, ECF271, ECF26, ECF40, ECF56, ECF33|
|Reactive oxygen species-inducible ECF σ factors of Bradyrhizobium japonicum.||PloS one||2012||N. Masloboeva, L. Reutimann, P. Stiefel, R. Follador, N. Leimer, H. Hennecke, S. Mesa, H. Fischer||PubMed: 22916258||ECF16, ECF33|
|Design of orthogonal genetic switches based on a crosstalk map of σs, anti-σs, and promoters.||Molecular systems biology||2013||V. Rhodius, T. Segall-Shapiro, B. Sharon, A. Ghodasara, E. Orlova, H. Tabakh, D. Burkhardt, K. Clancy, T. Peterson, C. Gross, C. Voigt||PubMed: 24169405||ECF22, ECF27, ECF03, ECF21, ECF39, ECF25, ECF26, ECF42, ECF38, ECF14, ECF29, ECF33, ECF281, ECF290, ECF291|