General description: Members of ECF294 are present in Proteobacteria (96.15%) and Acidobacteria (3.95%). Members of this group contain a ~120aa C-terminal extension with a SnoaL-like domain (Pfam: SnoaL_2). SnoaL is a small polyketide cyclase that catalyzes closure steps of the synthesis of polyketide antibiotics in Streptomyces spp. (Sultana et al., 2004). However, it has also been observed as part of ECF σ factors from original groups ECF56 and ECF41 (Huang, Pinto, Fritz, & Mascher, 2015; Staroń et al., 2009).
Genomic context conservation: The genetic context of members of ECF294 is not conserved beyond the ECF coding sequence.
Promoter motif conservation: Predicted target promoter motifs are not conserved among different subgroups.
Summary: Members of ECF294 compose a new group of SnoaL-like C-terminal extension σ factors with no homology to any original group. As in the case of ECF56 and ECF41 from the original ECF classification (Huang et al., 2015; Staroń et al., 2009), this C-terminal extension could be regulating ECF activity given the absence of any putative AS factor in the genetic context of members of ECF294.
Number of representative ECFs: 261
Number of non-redundant ECFs: 300
Sequences with C-terminal extension: 100.00%
Sequences with N-terminal extension: 0.33%
Overrepresented phylum: Proteobacteria [99.23%]
|Structure of the polyketide cyclase SnoaL reveals a novel mechanism for enzymatic aldol condensation.||The EMBO journal||2004||A. Sultana, P. Kallio, A. Jansson, J. Wang, J. Niemi, P. Mäntsälä, G. Schneider||PubMed: 15071504||ECF56, ECF294|
|The third pillar of bacterial signal transduction: classification of the extracytoplasmic function (ECF) sigma factor protein family.||Molecular microbiology||2009||A. Staroń, H. Sofia, S. Dietrich, L. Ulrich, H. Liesegang, T. Mascher||PubMed: 19737356||ECF103, ECF21, ECF123, ECF51, ECF39, ECF281, ECF102, ECF130, ECF122, ECF291, ECF15, ECF242, ECF22, ECF285, ECF106, ECF27, ECF31, ECF240, ECF114, ECF16, ECF38, ECF41, ECF105, ECF116, ECF111, ECF03, ECF239, ECF42, ECF294, ECF17, ECF11, ECF29, ECF235, ECF293, ECF118, ECF265, ECF30, ECF23, ECF14, ECF249, ECF18, ECF115, ECF290, ECF25, ECF121, ECF02, ECF120, ECF289, ECF28, ECF243, ECF19, ECF43, ECF107, ECF12, ECF32, ECF36, ECF292, ECF286, ECF271, ECF26, ECF40, ECF56, ECF33|
|Environmental Sensing in Actinobacteria: a Comprehensive Survey on the Signaling Capacity of This Phylum.||Journal of bacteriology||2015||X. Huang, D. Pinto, G. Fritz, T. Mascher||PubMed: 25986905||ECF122, ECF56, ECF118, ECF128, ECF51, ECF52, ECF132, ECF53, ECF123, ECF125, ECF54, ECF130, ECF131, ECF218, ECF294, ECF48|