General description: Proteins from ECF292 appear in Actinobacteria, mainly in Micrococcales and Corynebacteriales.
Anti-σ factor: Genomic contexts of members of ECF292 contain an average of 2.08 soluble proteins (94.32%) from Asp23 family (Pfam: Asp23), which is one of the most abundant proteins S. aureus and is related with cell envelope homeostasis (Müller et al., 2014). The BLAST search recognizes proteins from the Asp23 family as AS factors since the ungrouped putative AS factor 1534 (YP_118547 from Nocardia farcinica) contains this domain in C-terminus (Staroń et al., 2009). Two different Asp23 proteins appear in our analysis, the ones with Asp23 domain and longer versions with an N-terminal extension whose length is subgroup-specific (~100 aa in ECF292s1 to ~20 in ECF292s2) and are encoded in position +1. Whether Asp23 family proteins are AS factors is unknown, but it could be possible since their deletion leads to upregulation of the cell envelope stress genes (Müller et al., 2014). Asp23 from S. aureus is attached to the membrane via DUF322 family protein (AmaP) (Müller et al., 2014). Interestingly, Asp23 proteins are also putative regulators of ECF286.
Genomic context conservation: In ECF292s4 there is also a conserved DUF2273-containing protein in -3.
Promoter motif conservation: Promoter motifs are not conserved.
Summary: ECF292 might be regulated by a new kind of soluble AS factor that belongs to the Asp23 family. This group could be involved in cell envelope stress response.
Number of representative ECFs: 388
Number of non-redundant ECFs: 391
Sequences with C-terminal extension: 0.51%
Sequences with N-terminal extension: 7.42%
Overrepresented order: Micrococcales [56.96%]
|The third pillar of bacterial signal transduction: classification of the extracytoplasmic function (ECF) sigma factor protein family.||Molecular microbiology||2009||A. Staroń, H. Sofia, S. Dietrich, L. Ulrich, H. Liesegang, T. Mascher||PubMed: 19737356||ECF114, ECF31, ECF22, ECF12, ECF27, ECF122, ECF121, ECF56, ECF03, ECF21, ECF23, ECF02, ECF41, ECF15, ECF107, ECF111, ECF39, ECF19, ECF25, ECF17, ECF26, ECF118, ECF11, ECF16, ECF42, ECF38, ECF103, ECF36, ECF28, ECF51, ECF115, ECF40, ECF14, ECF29, ECF123, ECF33, ECF102, ECF105, ECF106, ECF116, ECF130, ECF18, ECF235, ECF120, ECF239, ECF240, ECF242, ECF243, ECF249, ECF265, ECF271, ECF281, ECF285, ECF286, ECF289, ECF290, ECF291, ECF292, ECF293, ECF294, ECF30, ECF32, ECF43|
|Deletion of membrane-associated Asp23 leads to upregulation of cell wall stress genes in Staphylococcus aureus.||Molecular microbiology||2014||M. Müller, S. Reiß, R. Schlüter, U. Mäder, A. Beyer, W. Reiß, J. Marles-Wright, R. Lewis, H. Pförtner, U. Völker, K. Riedel, M. Hecker, S. Engelmann, J. Pané-Farré||PubMed: 25074408||ECF286, ECF292|