Special features: ECF29 contains a conserved RCE/D motif in a short (~30aa) C-terminal extension that could be in charge of the modulation of its activity, either participating in sensing or repression of the ECF σ factor. Since the ECFs from ECF29 are soluble, they could sense cytoplasmic signals that could be related to ROS, disulfide or heavy metal stress.
Anti-σ factor: Proteins from ECF29 do not contain any evident putative AS factor.
Genomic context conservation: No conserved elements were found in the genetic context of members of ECF29.
Promoter motif conservation: Predicted target promoters of ECF29 usually contain GGGAACCT in -25 and CATCCAAT in -10, in agreement with original ECF29 (Rhodius et al., 2013). In subgroups ECF29s18 and ECF29s14 from Bacteroidetes, the putative target promoter motifs are TTAGAT (-35) GTTACA in -10.
Summary: ECF29 might be regulated by a short C-terminal extension with an RCE/D motif. The predicted target promoter motifs are conserved, indicating the positive autoregulation of ECFs from ECF29. Functional characterization of members of ECF29 would shed light on the function of members of ECF29.
Number of representative ECFs: 638
Number of non-redundant ECFs: 753
Sequences with C-terminal extension: 4.38%
Sequences with N-terminal extension: 3.05%
Overrepresented phylum: Proteobacteria [90.28%]
|The third pillar of bacterial signal transduction: classification of the extracytoplasmic function (ECF) sigma factor protein family.||Molecular microbiology||2009||A. Staroń, H. Sofia, S. Dietrich, L. Ulrich, H. Liesegang, T. Mascher||PubMed: 19737356||ECF103, ECF21, ECF123, ECF51, ECF39, ECF281, ECF102, ECF130, ECF122, ECF291, ECF15, ECF242, ECF22, ECF285, ECF106, ECF27, ECF31, ECF240, ECF114, ECF16, ECF38, ECF41, ECF105, ECF116, ECF111, ECF03, ECF239, ECF42, ECF294, ECF17, ECF11, ECF29, ECF235, ECF293, ECF118, ECF265, ECF30, ECF23, ECF14, ECF249, ECF18, ECF115, ECF290, ECF25, ECF121, ECF02, ECF120, ECF289, ECF28, ECF243, ECF19, ECF43, ECF107, ECF12, ECF32, ECF36, ECF292, ECF286, ECF271, ECF26, ECF40, ECF56, ECF33|
|Design of orthogonal genetic switches based on a crosstalk map of σs, anti-σs, and promoters.||Molecular systems biology||2013||V. Rhodius, T. Segall-Shapiro, B. Sharon, A. Ghodasara, E. Orlova, H. Tabakh, D. Burkhardt, K. Clancy, T. Peterson, C. Gross, C. Voigt||PubMed: 24169405||ECF22, ECF27, ECF03, ECF21, ECF39, ECF25, ECF26, ECF42, ECF38, ECF14, ECF29, ECF33, ECF281, ECF290, ECF291|