Anti-σ factor: Members of ECF289 encode a putative AS factor in position +1. This putative AS factor contains a von Willebrand domain fused to a DUF3520 in C-terminal and harbors one transmembrane helix (87.34%). In ECF289s6 the N-terminal domain ASD is truncated even though the rest of the protein is similar other putative AS factors from ECF289.
Promoter motif conservation: Predicted target promoter motifs are conserved and contain CCCAAGGA in -35 and CGTCTxA in -10. In subgroups ECF289s2 and ECF289s4 the up-element (AAAATTT) is more conserved than the -10 element. The overall conservation of the predicted binding motifs indicates that members of ECF289 self-regulate their transcription.
Summary: ECF289 is regulated by putative AS factors encoded in +1 with a DUF3520 fused to a von Willebrand domain. These AS factors are bound to the membrane by a single transmembrane helix. Original ECF20 was found close to von Willebrand factors, but they were not identified as AS factors (Staroń et al., 2009). New experiments will determine if these proteins are functioning as AS factors.
Number of representative ECFs: 344
Number of non-redundant ECFs: 343
Sequences with C-terminal extension: 0.00%
Sequences with N-terminal extension: 0.29%
Overrepresented class: Alphaproteobacteria [95.64%]
|The third pillar of bacterial signal transduction: classification of the extracytoplasmic function (ECF) sigma factor protein family.||Molecular microbiology||2009||A. Staroń, H. Sofia, S. Dietrich, L. Ulrich, H. Liesegang, T. Mascher||PubMed: 19737356||ECF103, ECF21, ECF123, ECF51, ECF39, ECF281, ECF102, ECF130, ECF122, ECF291, ECF15, ECF242, ECF22, ECF285, ECF106, ECF27, ECF31, ECF240, ECF114, ECF16, ECF38, ECF41, ECF105, ECF116, ECF111, ECF03, ECF239, ECF42, ECF294, ECF17, ECF11, ECF29, ECF235, ECF293, ECF118, ECF265, ECF30, ECF23, ECF14, ECF249, ECF18, ECF115, ECF290, ECF25, ECF121, ECF02, ECF120, ECF289, ECF28, ECF243, ECF19, ECF43, ECF107, ECF12, ECF32, ECF36, ECF292, ECF286, ECF271, ECF26, ECF40, ECF56, ECF33|