Anti-σ factor: Proteins from ECF286 are encoded together with several soluble proteins from Asp23 family (Pfam: Asp23), which is one of the most abundant proteins of S. aureus and is related with cell envelope homeostasis (Müller et al., 2014). Deletion of Asp23 in S. aureus leads to upregulation of the cell wall stress genes (Müller et al., 2014); therefore, this protein could function as AS factor.
Genomic context conservation: Other than Asp23, the genetic context of ECF286s4 contains a conserved RibD C-terminal domain in -1 and an AFG1-like ATPase in -2.
Promoter motif conservation: Predicted target promoter motifs are conserved and contain a conserved GAC in -35 and TC in -10.
Summary: In contrast to original ECF20 (Staroń et al., 2009), members of ECF286 do not seem to be regulated by canonical AS factors. Instead, they contain several Asp23-like proteins, indicating that members of ECF286 are involved in cell envelope homeostasis as in the case of Asp23 in S. aureus (Müller et al., 2014). Provided that these Asp23 proteins function as AS factors of members of ECF286, it remains unclear why each ECF from ECF283 encodes an average of 3.33 Asp23 proteins in its genetic neighborhood.
Number of representative ECFs: 456
Number of non-redundant ECFs: 410
Sequences with C-terminal extension: 0.00%
Sequences with N-terminal extension: 3.66%
Overrepresented order: Streptomycetales [50.88%]
|The third pillar of bacterial signal transduction: classification of the extracytoplasmic function (ECF) sigma factor protein family.||Molecular microbiology||2009||A. Staroń, H. Sofia, S. Dietrich, L. Ulrich, H. Liesegang, T. Mascher||PubMed: 19737356||ECF103, ECF21, ECF123, ECF51, ECF39, ECF281, ECF102, ECF130, ECF122, ECF291, ECF15, ECF242, ECF22, ECF285, ECF106, ECF27, ECF31, ECF240, ECF114, ECF16, ECF38, ECF41, ECF105, ECF116, ECF111, ECF03, ECF239, ECF42, ECF294, ECF17, ECF11, ECF29, ECF235, ECF293, ECF118, ECF265, ECF30, ECF23, ECF14, ECF249, ECF18, ECF115, ECF290, ECF25, ECF121, ECF02, ECF120, ECF289, ECF28, ECF243, ECF19, ECF43, ECF107, ECF12, ECF32, ECF36, ECF292, ECF286, ECF271, ECF26, ECF40, ECF56, ECF33|
|Deletion of membrane-associated Asp23 leads to upregulation of cell wall stress genes in Staphylococcus aureus.||Molecular microbiology||2014||M. Müller, S. Reiß, R. Schlüter, U. Mäder, A. Beyer, W. Reiß, J. Marles-Wright, R. Lewis, H. Pförtner, U. Völker, K. Riedel, M. Hecker, S. Engelmann, J. Pané-Farré||PubMed: 25074408||ECF286, ECF292|