General description: Members of ECF285 are present in Proteobacteria (100%).
Anti-σ factor: Members of ECF285 encode 2-transmembrane helix proteins (67.39%) with a DUF2892 in -1. These proteins could be a new type of AS factor with no homology to AS factors from the original library (Staroń et al., 2009).
Genomic context conservation: Genetic context conservation extends to a DUF692 (+2 of ECF285s5), a putative DNA-binding domain (Pfam: DUF2093) (+3 of ECF285s5), a RNA 2'-O ribose methyltransferase (ECF285s5), a PBP (Pfam: PBP_like_2) that might bind to phosphatidylethanolamine and have a role in membrane signal transduction (Banfield, Barker, Perry, & Brady, 1998) (ECF285s5), an exonuclease (Pfam: RNase_T) (ECF285s5), the periplasmic component of an ABC transporter (ECF285s5) and a PhoU, mainly as part of the putative AS factor in position -1, but also possibly involved in phosphate uptake (ECF285s5).
Promoter motif conservation: Predicted target promoter motifs of members of ECF285 contain AA in -35 and CCTCT in -10.
Summary: members of ECF285 might be regulated by a new type of single-transmembrane helix-containing AS factors encoded in position -1.
Number of representative ECFs: 214
Number of non-redundant ECFs: 217
Sequences with C-terminal extension: 0.46%
Sequences with N-terminal extension: 1.84%
Overrepresented class: Betaproteobacteria [43.93%]
|Function from structure? The crystal structure of human phosphatidylethanolamine-binding protein suggests a role in membrane signal transduction.||Structure (London, England : 1993)||1998||M. Banfield, J. Barker, A. Perry, R. Brady||PubMed: 9782050||ECF285|
|The third pillar of bacterial signal transduction: classification of the extracytoplasmic function (ECF) sigma factor protein family.||Molecular microbiology||2009||A. Staroń, H. Sofia, S. Dietrich, L. Ulrich, H. Liesegang, T. Mascher||PubMed: 19737356||ECF103, ECF21, ECF123, ECF51, ECF39, ECF281, ECF102, ECF130, ECF122, ECF291, ECF15, ECF242, ECF22, ECF285, ECF106, ECF27, ECF31, ECF240, ECF114, ECF16, ECF38, ECF41, ECF105, ECF116, ECF111, ECF03, ECF239, ECF42, ECF294, ECF17, ECF11, ECF29, ECF235, ECF293, ECF118, ECF265, ECF30, ECF23, ECF14, ECF249, ECF18, ECF115, ECF290, ECF25, ECF121, ECF02, ECF120, ECF289, ECF28, ECF243, ECF19, ECF43, ECF107, ECF12, ECF32, ECF36, ECF292, ECF286, ECF271, ECF26, ECF40, ECF56, ECF33|