General description: Proteins of ECF249 have homology to original groups ECF04 (2.63%), ECF10 (2.19%), ECF20 (0.44%) and ECF52 (0.44%) and they are present mainly in Firmicutes (41.81%), Bacteroidetes (27.68%), Acidobacteria (9.6%), Actinobacteria (4.52%), Chlorobi (4.52%), Planctomycetes (7.91%), Proteobacteria (0.56%) and Verrucomicrobia (3.39%).
Anti-σ factor: Members of ECF249 are regulated by AS factors in position +1 (except ECF249s6) and contain one (38.21%) or two (43.21%) transmembrane helices (only in ECF249s1). Putative AS factors of ECF249s14, which has homology to the original group ECF52, are fused to the ECF.
Genomic context conservation: Other conserved proteins include several cytochrome proteins, enzymes for the synthesis of tryptophan and proteins with other catalytic activities conserved in ECF249s1, and an EF-hand domain (Ca2+ binding) and a SCO1/SenC protein (required for the optimal cytochrome c oxidase activity) in ECF249s3.
Promoter motif conservation: Predicted target promoter motifs of the contain CGCAACTTT in -35 and GTT in -10.
Summary: ECF249 combines several original groups. AS factors of ECF249 contain one TM helix in most of the subgroups. Promoter motifs are conserved, which indicates that members of ECF249 are autoregulated.
Number of representative ECFs: 522
Number of non-redundant ECFs: 456
Sequences with C-terminal extension: 3.29%
Sequences with N-terminal extension: 2.63%
Overrepresented phylum: Firmicutes [45.79%]
|The third pillar of bacterial signal transduction: classification of the extracytoplasmic function (ECF) sigma factor protein family.||Molecular microbiology||2009||A. Staroń, H. Sofia, S. Dietrich, L. Ulrich, H. Liesegang, T. Mascher||PubMed: 19737356||ECF103, ECF21, ECF123, ECF51, ECF39, ECF281, ECF102, ECF130, ECF122, ECF291, ECF15, ECF242, ECF22, ECF285, ECF106, ECF27, ECF31, ECF240, ECF114, ECF16, ECF38, ECF41, ECF105, ECF116, ECF111, ECF03, ECF239, ECF42, ECF294, ECF17, ECF11, ECF29, ECF235, ECF293, ECF118, ECF265, ECF30, ECF23, ECF14, ECF249, ECF18, ECF115, ECF290, ECF25, ECF121, ECF02, ECF120, ECF289, ECF28, ECF243, ECF19, ECF43, ECF107, ECF12, ECF32, ECF36, ECF292, ECF286, ECF271, ECF26, ECF40, ECF56, ECF33|