General description: ECF240 is the main group with homology to original ECF10 (82.76%). Proteins of this group are present in Bacteroidetes (99.89%). The internal clade formed by ECF240s106, ECF240s155 and ECF240s180 (family Flavobacteriae) contains a conserved ~55aa C-terminal extension. Several subgroups contain proteins with transmembrane helices (up to an average of 0.89 TMH per protein).
Anti-σ factor and regulation: Likewise original ECF10 (Staroń et al., 2009), genetic contexts of members of ECF240 encode a FecR-like AS factor in +1, a TonB-dependent outer membrane receptor in +2 (Staroń et al., 2009) and an outer-membrane carbohydrate-binding protein in +3 (Pfam: SusD_RagB: 84.20%; Pfam: SusD-like_2/3: 98.73%; Pfam: Reg_prop: 106.79%). The AS factor is fused to a DUF4974, as in the case of other FecI-like AS factors, and it contains one transmembrane helix (87.7%). In some subgroups, this putative operon is inverted in a way that the carbohydrate-binding protein is encoded -3, the TonB-dependent receptor in -2 and the AS factor in -1 (ECF240s1, ECF240s22).
Genomic context conservation: Other conserved proteins encoded in the neighborhood of members of ECF240 are a glycosyl hydrolase family 92 (-1 and +2 of ECF240s7) and a lysine exporter LysO (ECF240s4).
Promoter motif conservation: Predicted target promoter motifs are not conserved, in agreement with the lack of autoregulation associated with FecI-like groups (Staroń et al., 2009).
Summary: ECF240 maintains the characteristics of the original group ECF10 (Staroń et al., 2009). ECF240 is not autoregulated, and its function might be related to carbohydrate scavenging and degradation in Bacteroidetes, one of the most critical components of gut microbiota.
Number of representative ECFs: 4028
Number of non-redundant ECFs: 4112
Sequences with C-terminal extension: 0.54%
Sequences with N-terminal extension: 2.55%
Overrepresented phylum: Bacteroidetes [99.73%]
|The third pillar of bacterial signal transduction: classification of the extracytoplasmic function (ECF) sigma factor protein family.||Molecular microbiology||2009||A. Staroń, H. Sofia, S. Dietrich, L. Ulrich, H. Liesegang, T. Mascher||PubMed: 19737356||ECF103, ECF21, ECF123, ECF51, ECF39, ECF281, ECF102, ECF130, ECF122, ECF291, ECF15, ECF242, ECF22, ECF285, ECF106, ECF27, ECF31, ECF240, ECF114, ECF16, ECF38, ECF41, ECF105, ECF116, ECF111, ECF03, ECF239, ECF42, ECF294, ECF17, ECF11, ECF29, ECF235, ECF293, ECF118, ECF265, ECF30, ECF23, ECF14, ECF249, ECF18, ECF115, ECF290, ECF25, ECF121, ECF02, ECF120, ECF289, ECF28, ECF243, ECF19, ECF43, ECF107, ECF12, ECF32, ECF36, ECF292, ECF286, ECF271, ECF26, ECF40, ECF56, ECF33|