Anti-σ factor: As members of original ECF47, ECF49 and ECF50 (Huang, Pinto, Fritz, & Mascher, 2015), members of ECF218 contain a putative AS factor with one transmembrane helix (74.33%) in position +1. This AS factor contains a RskA-like domain, but in most of the cases, there is no Pfam domain describing it. In subgroup ECF218s8, the AS factor contains a DUF4349 with two transmembrane helices.
Genomic context conservation: Genetic context conservation only extends beyond the AS factor coding sequence in ECF218s8, which contains an EPSP synthase and an aconitate hydratase.
Studied members: There is one described member of this group, Sig(MibX) from Microbispora corallina, involved in lantibiotic production (Foulston & Bibb, 2011). This protein is not included in the ECF library since its genome annotation was missing.
Promoter motif conservation: Different predicted target promoter motifs are possible depending on the subgroup. For instance, members of ECF218s41, ECF218s28 and ECF218s33 contain TGCAAG in -35 and CGACA in -10; members of ECF218s11 and ECF218s57 contain GAAAT in -35 and ACAT in -10; members of ECF218s16 and ECF218s5 contain GTAACGC in -35 and CCAAGTA in -10.
Number of representative ECFs: 2804
Number of non-redundant ECFs: 3037
Sequences with C-terminal extension: 5.40%
Sequences with N-terminal extension: 1.42%
Overrepresented phylum: Actinobacteria [99.82%]
|Feed-forward regulation of microbisporicin biosynthesis in Microbispora corallina.||Journal of bacteriology||2011||L. Foulston, M. Bibb||PubMed: 21478362||ECF218|
|Environmental Sensing in Actinobacteria: a Comprehensive Survey on the Signaling Capacity of This Phylum.||Journal of bacteriology||2015||X. Huang, D. Pinto, G. Fritz, T. Mascher||PubMed: 25986905||ECF122, ECF56, ECF118, ECF128, ECF51, ECF52, ECF132, ECF53, ECF123, ECF125, ECF54, ECF130, ECF131, ECF218, ECF294, ECF48|