Genetic context conservation: There are no putative AS factors present for members of ECF122. Instead, all subgroups of ECF122 contain a conserved 6-O-methylguanine DNA methyltransferase involved in DNA repair encoded in position +1 (an average of 1.26 ribonuclease-like domains and 1.41 DNA binding domains per ECF). Whereas this is the only conserved function in the context of proteins from subgroups ECF122s1, ECF122s2, and ECF122s3, proteins from the outermost subgroup, ECF122s4, are also associated with a glycerophosphoryl diester phosphodiesterase (-1), an histidine kinase-like ATPase domain also known as GHKL domain from Gyrase, Hsp90, Histidine Kinase, MutL since they share a structurally related ATPase domain (Staroń et al., 2009) (-2), one or two copies of the glycosyltransferase family 2 domain involved in transferring sugar from/to a variety of substracts (-4), three repeats of the glycosyltransferase domain from family 87 involved in the synthesis of glycolipids in mycobacteria (Wilson & Lamont, 2006) and proteins containing the domain UPF0016 suggested to function as calcium transporters (Huang, Pinto, Fritz, & Mascher, 2015).
Promoter motif conservation: The predicted target promoter motifs share the pattern CTCAC in -35 and CGTCTAC in -10 in ECF122s1, ECF122s2, and ECF122s3, whereas the promoter motif of ECF122s4 is not conserved. The information content of these motifs is reduced.
Summary: All in all, the proteins from ECF122 seem to be involved in DNA damage repair. Moreover, genetic neighborhoods of subgroup ECF122s4 contains conserved proteins related to glycolipid metabolism. A possible reason is that members of ECF122s4 evolved in a different range of bacteria that the rest of the members of ECF122. Supporting this notion, members of ECF122s4 appear only in families Intrasporangiaceae (90%) and Nocardioidaceae (10%), whereas remaining subgroups have a different family composition (at least six families in a subgroup).
Number of representative ECFs: 495
Number of non-redundant ECFs: 559
Sequences with C-terminal extension: 0.18%
Sequences with N-terminal extension: 0.36%
Overrepresented order: Corynebacteriales [52.32%]
|GHKL, an emergent ATPase/kinase superfamily.||Trends in biochemical sciences||2000||R. Dutta, M. Inouye||PubMed: 10637609||ECF122|
|PimE is a polyprenol-phosphate-mannose-dependent mannosyltransferase that transfers the fifth mannose of phosphatidylinositol mannoside in mycobacteria.||The Journal of biological chemistry||2006||Y. Morita, C. Sena, R. Waller, K. Kurokawa, M. Sernee, F. Nakatani, R. Haites, H. Billman-Jacobe, M. McConville, Y. Maeda, T. Kinoshita||PubMed: 16803893||ECF122|
|The third pillar of bacterial signal transduction: classification of the extracytoplasmic function (ECF) sigma factor protein family.||Molecular microbiology||2009||A. Staroń, H. Sofia, S. Dietrich, L. Ulrich, H. Liesegang, T. Mascher||PubMed: 19737356||ECF103, ECF21, ECF123, ECF51, ECF39, ECF281, ECF102, ECF130, ECF122, ECF291, ECF15, ECF242, ECF22, ECF285, ECF106, ECF27, ECF31, ECF240, ECF114, ECF16, ECF38, ECF41, ECF105, ECF116, ECF111, ECF03, ECF239, ECF42, ECF294, ECF17, ECF11, ECF29, ECF235, ECF293, ECF118, ECF265, ECF30, ECF23, ECF14, ECF249, ECF18, ECF115, ECF290, ECF25, ECF121, ECF02, ECF120, ECF289, ECF28, ECF243, ECF19, ECF43, ECF107, ECF12, ECF32, ECF36, ECF292, ECF286, ECF271, ECF26, ECF40, ECF56, ECF33|
|Molecular evolution of a novel family of putative calcium transporters.||PloS one||2014||D. Demaegd, A. Colinet, A. Deschamps, P. Morsomme||PubMed: 24955841||ECF122|
|Environmental Sensing in Actinobacteria: a Comprehensive Survey on the Signaling Capacity of This Phylum.||Journal of bacteriology||2015||X. Huang, D. Pinto, G. Fritz, T. Mascher||PubMed: 25986905||ECF122, ECF56, ECF118, ECF128, ECF51, ECF52, ECF132, ECF53, ECF123, ECF125, ECF54, ECF130, ECF131, ECF218, ECF294, ECF48|