Anti-σ factors: Likewise the original group ECF105 (Staroń et al., 2009), proteins from ECF105 are regulated by TM-bound ZAS factors encoded in position encoded in +1. These AS factor contain a single transmembrane helix (85.11%).
Genomic context conservation: The multiple transmembrane proteins encoded in positions +2 and +3 in original ECF105 (Staroń et al., 2009) were also found in new ECF105 (usually 9 (34.04%) and 3 (57.46%) transmembrane helices, respectively). Other proteins conserved in the genetic context of members of ECF105 are some enzymes in ECF105s1 involved in translation and amino acid metabolism: acetokinase, pyrroline-5-carboxylate reductase, tRNA synthetase class II and 3-hydroxyacyl-CoA dehydrogenase.
Promoter motif conservation: Predicted target promoter motifs are conserved. Two -35 elements contain TG(A/T)AGGG and the -10 element has CGTCTAT. The predicted target promoter motif expands the knowledge over members of original ECF105 (Staroń et al., 2009), which lacks a conserved target promoter motif.
Summary: ECF105 expands the number of proteins (from 4 in original ECF105 (Staroń et al., 2009) to 312 in new ECF105), predicts a target promoter motif but keeps the same genetic neighborhood as original group ECF105.
Number of representative ECFs: 382
Number of non-redundant ECFs: 527
Sequences with C-terminal extension: 0.00%
Sequences with N-terminal extension: 3.42%
Overrepresented class: Bacilli [67.80%]
|Adhesion to cellulose of the Gram-positive bacterium Ruminococcus albus involves type IV pili.||Microbiology (Reading, England)||2002||H. Rakotoarivonina, G. Jubelin, M. Hebraud, B. Gaillard-Martinie, E. Forano, P. Mosoni||PubMed: 12055307||ECF105|
|The third pillar of bacterial signal transduction: classification of the extracytoplasmic function (ECF) sigma factor protein family.||Molecular microbiology||2009||A. Staroń, H. Sofia, S. Dietrich, L. Ulrich, H. Liesegang, T. Mascher||PubMed: 19737356||ECF103, ECF21, ECF123, ECF51, ECF39, ECF281, ECF102, ECF130, ECF122, ECF291, ECF15, ECF242, ECF22, ECF285, ECF106, ECF27, ECF31, ECF240, ECF114, ECF16, ECF38, ECF41, ECF105, ECF116, ECF111, ECF03, ECF239, ECF42, ECF294, ECF17, ECF11, ECF29, ECF235, ECF293, ECF118, ECF265, ECF30, ECF23, ECF14, ECF249, ECF18, ECF115, ECF290, ECF25, ECF121, ECF02, ECF120, ECF289, ECF28, ECF243, ECF19, ECF43, ECF107, ECF12, ECF32, ECF36, ECF292, ECF286, ECF271, ECF26, ECF40, ECF56, ECF33|
|Type IV pili in Gram-positive bacteria.||Microbiology and molecular biology reviews : MMBR||2013||S. Melville, L. Craig||PubMed: 24006467||ECF31, ECF105|